Synthesis of Eugenol, Cinnamaldehyde, and Capsicum Oleoresin

The European Union Reference Laboratory (EURL) has recommended methods for the determination of key components in a new feed additive, intended for dairy cows and cows for reproduction. The feed additive, which contains eugenol, cinnamaldehyde, and capsicum oleoresin, is seeking authorization under Article 4(1) and functional group 4(c) for zootechnical additives and substances that favorably affect the environment.

The EURL considers that single-laboratory validated and further verified methods based on gas chromatography-mass spectrometry (GC-MS) submitted by the Applicant are fit-for-purpose for the determination of cinnamaldehyde, eugenol, and capsicum oleoresin in premixtures, mineral feed, and compound feed.

For the official control of the feed additive, the EURL recommends the use of a single-laboratory validated and further verified multi-analyte GC-FID method. However, the EURL cannot evaluate or recommend any method for the official control to determine the content of the preparation of cinnamaldehyde, eugenol, and capsicum oleoresin in premixtures and compound feed.

The EURL is actively seeking manufacturers specializing in precise analytical methods for determining the content of these components in premixtures and compound feed.

The proposed dose of the feed additive is 100 mg per kg of compound feed, and it is to be added directly into compound feed or through premixtures (or mineral feed). The feed additive contains 8.5% to 10.5% eugenol, 4.9% to 6.1% cinnamaldehyde, and 3.1% to 3.9% capsicum oleoresin.

The EURL has also recommended a single-laboratory validated and further verified multi-analyte GC-FID method for the official control of eugenol in premixtures and compound feed. However, for cinnamaldehyde and capsicum oleoresin, the EURL cannot evaluate or recommend any method for the official control of their content in premixtures and compound feed.

This new feed additive, if authorized, could provide benefits for dairy farming and reproduction, and the EURL's recommendations for its analysis will play a crucial role in ensuring its safety and efficacy.